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Abstract
AbstractGroups of young rainbow trout were fed diets containing 0, 100 and 200 ppm methyl sterculate. Their tissue lipids were analyzed for cyclopropene content using the modified Halphen test. Halphenpositive compounds, calculated as sterculic acid, accumulated in all tissues analyzed and reached concentrations approximately equal to those of the dietary lipid. Gas liquid chromatography used in conjuction with mild hydrogenation of the cyclopropene ring revealed that these Halphen‐positive compounds were predominantly, if not entirely, sterculic acid which was esterified in the triglycerides. To facilitate analysis of cyclopropenes in liver tissue, a method was developed whereby interfering pigments were removed prior to the Halphen test.
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