Abstract
Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.
Highlights
The ability of a device to interact with biological systems is often driven by the surface chemistry and topography
The current and voltage measurements were consistent with previous descriptionsofofthe nonthermal helium plasma discharges at atmospheric pressure
Results are average of assay at 24 h from carcinoma (HeLa)
Summary
The ability of a device to interact with biological systems is often driven by the surface chemistry and topography. Technologies that enhance the adhesion, growth, and interaction of living cells on various device surfaces of pivotal importance. Using either vacuum plasma systems or cold atmospheric pressure plasma treatments creates a range of hydrophilic surface finishes that enhance cell adhesion [2,3,4]. The use of low temperature vacuum plasma devices to deposit carbonyl, carboxyl, and amine functionalities onto nonreactive hydrophobic surfaces is a well-established industrial practice and underpins the biocompatibility of disposable plastic plates, flasks, and bottles of the cell culture market [1,10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
