Abstract
Objective To establish the method for removing high-abundance proteins in serum proteomics research and evaluate the removal efficiency of the high-abundance proteins. Methods Human 14 Multiple Affinity Removal System of Agilent Technologies was applied to eliminate the high-abundance proteins in serum, such as albumin, IgG and α-l-antitrypsin, etc. The efficiency of removal was evaluated by 1-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results The method for removing serum high-abundance proteins was established. The protein lanes of SDS-PAGE map were clearer and some low-abundance proteins were visualized after depletion. Conclusion An effective method of removing high-abundance proteins in serum is established, which provids basis for further strategies in proteomics research. Key words: Serum; Proteomics; High-abundance protein; Low-abundance protein
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
