Depletion of IL-22 during culture enhanced antigen-driven IFN-γ production by CD4+T cells from patients with active TB

Abstract

In this study, we mainly investigated the expression of IFN-γ and IL-22-producing CD4+T cells, the relationship between the two cytokines in human tuberculosis. We showed that IFN-γ and IL-22 were induced by human mycobacterial infection. Besides, CD4+T cells expressing IFN-γ and IL-22 were significantly elevated following PPD stimulation. In addition, IFN-γ and IL-22 -expressing CD4+T cells were distinct from each other. We also found that there was a reciprocal relationship between IFN-γ and IL-22 production. The production of IL-22 was markedly enhanced in the absence of IFN-γ, also, the expression of IFN-γ was increased when the IL-22 signaling was inhibited by monoclonal anti-IL-22 mAb. Furthermore, data revealed that the detectable IL-22 affected the phynotype of IFN-γ+CD4+T cells. IL-22 induced up-regulation of CD27 expression in IFN-γ+CD4+T cells, which might further confirm that IL-22 had an effect on the response of IFN-γ. Thus, this work provides a previous unknown relationship between IFN-γ and IL-22 in human TB disease.