Depletion by preganglionic stimulation and post-stimulus recovery of large dense core vesicles in synaptic boutons of the cat superior cervical ganglion

Abstract

Large dense core vesicles (LDCV), a possible site for neuropeptide storage, were counted within synaptic boutons of the superior cervical ganglion of anaesthetized cats in the unstimulated condition and 2 h, 1 day or 10 days after 40 Hz 2 h stimulation of the cervical sympathetic trunk. The number of LDCV decreased markedly after stimulation. It was lowest at 2 h and then recovered slowly. Ten days post-train the number was close to, but still significantly lower than, control. This time course of recovery is much longer than for agranular vesicles number, which is known to be back to normal within 2 h of the end of a comparable stimulus train. The post-stimulus loss of LDCVs is consistent with the hypothesis that LDCVs are released by the nerve terminal. The slow recovery is consistent with the hypothesis of replenishment by axoplasmic transport.