Abstract
Non-invasive single cell analyses are increasingly required for the medicaldiagnostics of test substances or the development of drugs and therapies on the single celllevel. For the non-invasive characterisation of cells, impedance spectroscopy whichprovides the frequency dependent electrical properties has been used. Recently,microfludic systems have been investigated to manipulate the single cells and tocharacterise the electrical properties of embedded cells. In this article, the impedance ofpartially embedded single cells dependent on the cellular behaviour was investigated byusing the microcapillary. An analytical equation was derived to relate the impedance ofembedded cells with respect to the morphological and physiological change ofextracellular interface. The capillary system with impedance measurement showed afeasibility to monitor the impedance change of embedded single cells caused bymorphological and physiological change of cell during the addition of DMSO. By fittingthe derived equation to the measured impedance of cell embedded at different negativepressure levels, it was able to extrapolate the equivalent gap and gap conductivity betweenthe cell and capillary wall representing the cellular behaviour.
Highlights
In biological cell-based biotechnology, single cell analyses are increasingly required to understand the response and behaviour of individual cells to test substances (e.g. DNA, molecule, protein) or to develop the strategic therapies and drugs against disease on the single cell level [1]
As more as R3 contributes to Rdiff, the impedance measurement of embedded single cell reflects more the cellular behaviour related with the interfacial parameters li, g, and σ g
As one precondition for the development of sensor based on the impedance measurement at a cell/microhole interface, the impedance measurement on cells embedded in a microcapillary was investigated
Summary
In biological cell-based biotechnology, single cell analyses are increasingly required to understand the response and behaviour of individual cells to test substances (e.g. DNA, molecule, protein) or to develop the strategic therapies and drugs against disease on the single cell level [1]. Even though the microelectrode-based methods showed a possibility to distinguish the impedance of cells under different conditions, it was not able to interpret the measured data of single cell contacted with electrodes due to the high contribution of electrode impedance increasing with decrease of electrode size. This inherent problem of electrodebased method is a crucial obstacle to understand the interfacial behaviour of single cells from the measured impedance (e.g. ion channel activity, cellular adhesion, membrane integrity). The microhole-based method had a limitation for the interpretation due to the difficulty of observing the exact cellular morphology
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