Abstract
This study was conducted to determine DEPDC1 expression in hepatocelluar carcinomas (HCCs) and to reveal its potential role in diagnosis and prognosis of affected patients. DEPDC1 expression at the mRNA level was detected by quantitative real-time PCR (qRT-PCR) in 205 cases of HCC and paired adjacent normal liver tissues, and by semi-quantitative RT-PCR in 20 cases. Survival curves were obtained by using Kaplan-Meier method and Log-rank test. Independent predictors associated with regard to disease free survival (DFS) and overall survival (OS) were identified using the Cox proportional hazard model. High DEPDC1 mRNA levels were detected in 144 out of 205 cases (70.24%) of HCC, significantly associated with clinicopathological parameters, including tumor size (≥4cm), alpha-fetoprotein (≥100ng/ml), B-C of BCLC stage and recurrence. Kaplan-Meier survival analysis revealed that HCC patients with high DEPDC1 expression had poor OS and DFS. Multivariate analysis demonstrated that high DEPDC1 expression was an independent predictor of OS (HR=1.651; 95% 95%CI, 1.041- 2.617; p=0.033) and DFS (HR=1.583; 95%CI, 1.01- 2.483; p=0.045). Our results indicate DEPDC1 might be a novel diagnostic marker and an independent prognostic predictor for HCC patients.
Highlights
Hepatocelluar carcinoma (HCC), the sixth most prevalent cancer type, ranks the third leading cause of cancer-related mortality worldwide (Forner et al, 2012), especially in many countries of Asia (Fazeli et al, 2012)
The results showed that DEP domain containing 1 (DEPDC1) mRNA was significantly higher in 14 cases of HCC (Figure 1A)
The results showed that DEPDC1 mRNA was up-regulated in 144 cases (70.24%), but down-regulated in 51 cases (29.76%) compared with adjacent normal liver tissues
Summary
Hepatocelluar carcinoma (HCC), the sixth most prevalent cancer type, ranks the third leading cause of cancer-related mortality worldwide (Forner et al, 2012), especially in many countries of Asia (Fazeli et al, 2012). DEP domain is a globular domain that consists of approximately 90 amino acids, which was first identified in three proteins: D. melanogaster Dishevelled, C. elegans EGL-10 and mammalian Pleckstrin (Ballon et al, 2006). These proteins are involved in Wnt signaling (Sokol, 2000), G-protein coupled receptor signaling , and signaling in platelets and neutrophils (Kharrat et al, 1998), respectively. This study was conducted to determine DEPDC1 expression in hepatocelluar carcinomas (HCCs) and to reveal its potential role in diagnosis and prognosis of affected patients. Kaplan-Meier survival analysis revealed that HCC patients with high DEPDC1 expression had poor OS and DFS. Conclusions: Our results indicate DEPDC1 might be a novel diagnostic marker and an independent prognostic predictor for HCC patients
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