Abstract
The control of DNA synthesis by the addition of graded concentrations of thymidine to HeLa cell cultures inhibited by 5-fluorodeoxyuridine has been determined to be a regulation at the cellular level and not a population selection process. In infected cells the rate of of vaccinia virus DNA in the presence of FUDR is not a function of the thymidine concentration. Instead, suboptimal thymidine levels were utilized preferentially to support optimal viral DNA synthetic rates which ceased abruptly when depletion of thymidine occurred. When FUDR was added early in the infectious cycle, the reduced yield of infectious virus obtained was also formed at a maximal rate.
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