Abstract
1. 1. The presence of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) has been demonstrated in L cells grown in culture and its properties have been studied. 2. 2. This DNA polymerase has been purified approximately 15-fold with respect to the protein of the crude extracts. 3. 3. The activity of crude extracts as well as that of partially purified preparations has been shown to be almost entirely dependent on the presence of a denatured DNA primer. For full activity of the purified preparation, all four deoxyribonucleoside triphosphates and Mg 2+ were also necessary. 4. 4. When DNA synthesis in L cells was inhibited in vivo, the DNA polymerase activity of cell extracts increased. When the inhibition was reversed, and DNA synthesis was reinitiated, the activity of the enzyme decreased. 5. 5. The observed changes in the activity of DNA polymerase are postulated to be due to altered intracellular states of the enzyme.
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