Abstract
Abstract Replication of polydeoxyadenylate and polydeoxythymidylate catalyzed by the high molecular weight DNA polymerase proceeds only in the presence of a complementary oligodeoxynucleotide. The oligodeoxynucleotide initiator chain must be greater than 6 nucleotides for poly(dA) replication and greater than 5 nucleotides for poly(dT) replication. The initiator oligonucleotide is incorporated into product chains. When a multiplicity of initiator chains are present (more than 1 per template) then several chains grow simultaneously on each template. The structure and molecular weight distribution of the products may be predicted from the initiator to template ratio.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
