Abstract

Deoxycytidine Triphosphatase, an Enzyme Induced by Bacteriophage Infection

Highlights

  • In previous communications we have brirfly dcscribrd the properties of a dcoxycytidine triphosphate-cleaving enzyme which appears in Escherichia coli cells after infection with bacteriophage T2 [1, 2]

  • This paper presents in detail our studies on the deoxyribonucleic acid (DNA) polymerase system which led to the discovery of the dCTPase, studies on the partial purification and the properties of the latter enzyme, and studies on its appcrance in the system after phage infection

  • Lehman et al [19] have shown that if a preparation of the DN.4 polymerase of E. coli is incubated with a solution containing magnesium ion and a suitable DNA primer, synthesis of DNA proceeds only in the presence of four appropriate deoxynucleoside triphosphates, e.g. dCTP, dTTP, dATP, and dGTP

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Summary

Introduction

In previous communications we have brirfly dcscribrd the properties of a dcoxycytidine triphosphate-cleaving enzyme (deoxycytidine triphosphatase) which appears in Escherichia coli cells after infection with bacteriophage T2 [1, 2]. Since cytosine is not found in the deoxyribonucleic acid of T2 phage and is replaced by 5-hydroxymethylcytosine and a glucoside of this compound [3,4,5], it is probable that the dCTPase has an important role in tioo in effecting this change of composition of the deoxyribonucleic acid after phage infection This enzyme has been observed by Kornberg et al [6, 7], because of its property of destroying the dCTP formed by the dCMP kinase which presumably supplies this substratc for the synthesis of deoxyribonucleic acid of B. coli. This paper presents in detail our studies on the DNA polymerase system which led to the discovery of the dCTPase, studies on the partial purification and the properties of the latter enzyme, and studies on its appcrance in the system after phage infection

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