Abstract

The purpose of this study was to evaluate the effects of flavonoids and calcium phosphate ion clusters (CPIC) on dentin bonding stability. Seven experimental solutions were synthesized using icaritin (ICT), fisetin (FIS), silibinin (SIB), CPIC, and combinations of one of three flavonoids and CPIC (ICT + C, FIS + C, SIB + C). The experimental solutions were applied to demineralized dentin prior to the application of a universal adhesive. A group without any experimental solution served as a control. Dentin specimens pretreated with the experimental solutions were assayed using Fourier transform infrared (FTIR) spectroscopy. The microtensile bond strength (µTBS) and nanoleakage were evaluated at 24 h and after 10,000 thermocycles. FIS and ICT + C showed significantly higher µTBS than the control group at 24 h. CPIC, ICT + C, FIS + C, and SIB + C showed significantly higher µTBS than the control group after thermocycling. After thermocycling, silver infiltration into the hybrid layer and interfacial gaps was more noticeable in the control group than in the other groups. The FTIR spectra revealed the formation of apatitic minerals in the demineralized dentin in the flavonoid and CPIC combination groups. The pretreatment of demineralized dentin with flavonoids and CPIC improved dentin bonding stability. The flavonoid and CPIC combinations preserved dentin bond strength.

Highlights

  • Despite continuous advancements in dentin bonding systems, resin-dentin interfaces are still the most vulnerable part of adhesive restorations [1,2]

  • The purpose of this study was to evaluate the effects of flavonoids, calcium phosphate ion clusters (CPIC), and flavonoids combined with CPIC on dentin bonding stability through bond strength and nanoleakage assessments

  • We investigated the potential effects of flavonoids and CPIC on dentin bonding stability

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Summary

Introduction

Despite continuous advancements in dentin bonding systems, resin-dentin interfaces are still the most vulnerable part of adhesive restorations [1,2]. The longevity of adhesive restorations depends on the stability of the so-called hybrid layer, which consists of resin penetrating into the collagenous network of the dentin [3]. An incomplete infiltration leaves unprotected collagen and porosities in the hybrid layer. This phenomenon was termed “nanoleakage”, which can work as the pathway for the degradation of resin-dentin bonds over time. The degradation of the collagenous matrix at resin-dentin interfaces impairs dentin bonding stability, which results in a decrease in bond strength and increase in nanoleakage

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