Dental Tissue Engineering of EMPs on Human Dental Pulp Stem Cells

Abstract

The aim of this study was to isolate and characteristics human dental pulp stem cells (hDPSCs) derived from human third molar pulp. Another aim was to verify the expression with dentin sialoprotein (DSPP), dentin sialoprotein (DSP), dentin matrixprotein 1 (DMP-1) and vimentin. For characterisation, proliferation capacity, phenotypic properties, and differentiation characteristics were utilised. Stem cells isolated from hNDP were analysed by flow cytometry and immunocytochemistry. Cell line were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. The expression of DSPP, DSP, DMP-1and vimentin, as analyzed by Immunocytochemistry, increased after EMPs incubating for 5d. So exhibit multilineage differentiation properties. Thus, both EMPs are important during hDPSCs proliferation. During cytodifferentiation, EMPs are essential for the complete differentiation of odontoblasts by up-regulating the expression of DSPP, DSP, DMP-1 and vimentin. Stimulation of these cells by EMPs significantly increases alkaline phosphatase(ALP) activity. ALP activity was expressed as OD 405 nm/mg of protein A increase was observed in ALP activity of hDPSCs during the culturing of EMPs. According to the differentiation and proliferation potential of HDPSC was shown. A specific function for EMPs up-regulat in differentiation of hDPSCs was elucidated.