Abstract
High density L cell suspension cultures were previously shown to remain viable for prolonged periods of time and to exhibit stability of population and regulation of DNA synthesis and mitosis comparable to growth-inhibited, dense attached cultures of fibroblasts. The present data demonstrate that this similarity extends to the regulation of total protein and collagen synthesis in spite of a media pO 2 decline to virtually undetectable levels within 8 h after resuspension of the cells. Possible mechanisms accounting for this are discussed as well as evidence suggesting comparable pO 2 changes in the cellular microenvironment of dense attached cultures. It is concluded that, for the parameters so far investigated, cell to cell contact is not a universal prerequisite of density-dependent inhibition of growth.
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