Abstract
A thin-layer chromatographic method has been established for quantification of aloenin in aloe ( Aloe arborescens Mill.) pharmaceuticals. Chromatographic separation was performed on silica gel plates with ethyl acetate-ethanol (95%)-water, 20:3:1 ( v/v ), as mobile phase. The plates were scanned densitometrically at 365 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise — intra-day and inter-day RSD were 2.21% and 3.15%, respectively. Instrumental precision and repeatability for the method were 0.42 and 1.94 ( CV [%]), respectively. Accuracy was checked by measurement of recovery at three levels; average recovery was 97.86%. The method was used for analysis of aloenin in aloe juice, aloe tablets and aloe liquid extract for injections and was confirmed to be suitable for this purpose.
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