Abstract
Alternaria alternata is an important mycotoxin-producing species that occurs on cereals, sunflower seeds, oilseed rape, tomato, carrot, lentils, olives, various fruits, etc. A. alternata produces a number of mycotoxins, including alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), L-tenuazonic acid (TeA), and other less-toxic metabolites. The objective of this study was a toxicological characterization of A. alternata strains isolated from wheat (34 strains) and grapes (11 strains). All the A. alternata strains were cultured on rice to evaluate their toxigenic potential (incubated in the dark at 25°C for 21 days). An instrumental high-performance thinlayer chromatography (HPTLC) for the quantification of ALT, AOH, AME, and TeAin rice cultures has been developed. The mycotoxins were extracted with methanol and ammonium sulfate in water and the extracted solutions were further cleaned up by liquid-liquid extraction. The silica gel layers pre-coated with oxalic acid in methanol were used ...
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