Abstract
A densitometric TLC method has been developed for identification and quantification of fluoxetine and its impurities — (1 RS )-3-methylamino-1-phenylpropan-1-ol, N -methyl-3-phenylpropan-1-amine, and C-1, the last of which has not previously been analyzed. The compounds were separated on silica gel 60 F 254 HPTLC plates, with chloroform-methanol-25% ammonia, 45 + 4.5 + 0.5 ( v/v ), as mobile phase. UV and visible densitometric scanning were performed at λ = 260 and 530 nm, respectively, after visualization of the chromatograms with ninhydrin solution. The method enables good separation of the constituents ( R F = 0.20, 0.41, 0.52, and 0.67) with sufficiently high detection limit and recovery for the individual constituents.
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