Abstract
Summary Separation and quantitative analysis of quercetin glycosides in methanolic and aqueous extracts of Epilobii angustifolii herba have been achieved by HPTLC with densitometric detection. The compounds were separated on silica gel 60F254 HPTLC plates with ethyl acetate–formic acid–water, 68 + 2.5 + 3 (v/v), as mobile phase; densitometric detection of the flavonoids was performed at λ = 350 nm. The amounts of the compounds were calculated by use of regression equations obtained from calibration plots. The flavonoids were more abundant in the aqueous extract than in the methanolic extract. Quercetin glucuronide was the most abundant compound in both extracts (21.22 mg g–1 and 17.85 mg g–1, respectively). The method is rapid, easy, and selective, particularly for quercetin glucuronide analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
