Denitrogenation of carbazole by a novel strain Bacillus Clausii BS1 isolated from Egyptian Coke

Abstract

ABSTRACTIn this study, an effective enrichment technique was applied to isolate different bacterial strains with capabilities to denitrogenate carbazole (CAR), as a model compound for nitrogenous polyaromatic hydrocarbons (NPAHs). Twenty-four different CAR-denitrogenating bacterial strains were isolated from mineral coke samples. From all, a Gram +ve bacterial isolate designated BS1 showed a higher biodenitrogenation (BDN) efficiency relevant to the well-known biodenitrogenating bacterium strain Pseudomonas resinovorans CA10, recording 77.15 and 60.66% removal of 1000 ppm CAR with the production of 119.79 and 102.43 ppm anthranilic acid, and 121.19 and 90.33 ppm catechol, as by-products, respectively. BS1 was identified by 16S rDNA gene sequence analysis to be Bacillus clausii BS1 (NCBI GenBank Accession no. KF985981) with a similarity of 99.14%. The CAR-BDN pathway was also elucidated using high-performance liquid chromatography (HPLC) and gas chromatography mass spectrometry analysis (GC/MS), which proved further metabolism of catechol to muconic acid.