Denitrification rates in estuarine sediments of Ashtamudi, Kerala, India.

Abstract

Estuarine sediments are important sites for denitrification, which is microbially mediated reduction of nitrate to dinitrogen that also influences global climate change by co-production of nitrous oxide, a potent greenhouse gas. Physicochemical properties and nutrients of sediment samples that influence denitrification rate were studied in Ashtamudi estuarine sediments. They were pH, electrical conductivity (EC), salinity, nitrate-nitrogen (NO3--N), exchangeable ammonia (NH3--N), total kjeldahl nitrogen (TKN) and organic carbon (Corg). Sediment samples were collected from six stations during summer, monsoon of 2013 and 13 stations from monsoon 2014 and summer 2015. The sedimentary denitrification potential ranged from 0.49 ± 0.05 to 4.85 ± 0.782mmol N2O m-2 h-1. Maximum denitrification was observed in S4, which is attributed to a local anthropogenic source coupled with intense rainfall episode preceding the sampling season of monsoon 2013. However, this trend was not repeated in the subsequent monsoon samples. This shows that in Ashtamudi, monsoonal effects do not influence sedimentary denitrification. Among the various environmental variables, NO3--N, Corg and NH3-N were the key factors that influence denitrification in the Ashtamudi estuarine sediments. Among these key factors, NO3--N was the limiting factor for denitrification, and hence, it is of prime importance to understand the source of NO3--N that fuel denitrification in the sediments. In Ashtamudi, the concentration of NO3--N in overlying water was very less, which suggests reduced nitrogen yield in the estuary from the fluvial input of Kallada River and agricultural runoff. Sedimentary NO3--N correlated with denitrification which reveals that denitrification is coupled with nitrification in the sediments. This is further explained by the fact that NH3-N positively correlated with denitrification. The anoxic sediments were the source of ammonia for nitrous oxide production by nitrogen mineralisation. Also, the Corg in sediment samples were sufficient to support denitrification and Corg was an important factor favouring but not limiting denitrification. The results of sediment denitrification in Ashtamudi can be a model for tropical estuaries experiencing unpredictable rainfall as well as high temperature than temperate systems.