Denitrification as a Sink for Dissolved Nitrous Oxide in a Freshwater Riparian Fen

Abstract

AbstractDenitrification as a sink of dissolved nitrous oxide (N2O) was investigated in a freshwater riparian fen. In a 15‐m transect extending from the hillslope and into the fen the groundwater concentrations of nitrate (NO−3) declined from 1.8 mM NO−3 (25 mg L−1) to less than 0.01 mM NO−3, dissolved oxygen (O2) and nitrous oxide (N2O) declined from approximately 110 µM O2 (3.5 mg O2 L−1) and 4.0 µM N2O‐N (56 µg N L−1), respectively, to zero and the dissolved N2 concentration increased by 589 µM N2‐N (8.2 mg N L−1). The NO−3 reduction was 0.42 µM cm−3 d−1 or 7.71 µM cm−2 d−1 in sediment columns with continuous upward groundwater flow through the sediment. Concomitant with NO−3 reduction, N2O was produced at a rate of 54.4 nM N2O‐N cm−2 d−1 in this same 18‐cm narrow sediment zone. However, the N2O produced was subsequently reduced at the same rate closer to the sediment surface. In 15NO−3 experiments on chloramphenicol‐treated anaerobic sediment slurries, the denitrifying enzyme activity (DEA) was estimated to be 118 ± 16.7 nmol N (N2‐N + N2O‐N) g fresh weight 1−1 d−1, of which 36% accumulated as N2O. Thus, in this permanently water‐covered riparian fen, denitrification served as a sink for both the dissolved N2O in groundwater recharging the fen and the N2O produced within the riparian sediment.