Denitrification and dissimilatory nitrate reduction to ammonium in freshwater lakes of the Eastern Plain, China: Influences of organic carbon and algal bloom

Abstract

Denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) are critical dissimilatory nitrate reduction pathways that determine nitrogen (N) removal and internal recycling in aquatic environments. However, the relative important of DNRA, and the influences of environmental factors on DNF and DNRA, have not been widely studied in freshwater lakes. In our study, we used N isotope-tracing to investigate the potential rates of DNF and DNRA in 27 lakes from the Eastern Plain Lake Zone (EPL), China. In the EPL lakes, DNF was the dominant nitrate reduction process, however DNRA was still important, accounting for around 4.3%–21.9% of total nitrate reduction. The sediment organic carbon was the primary factor controlling the rates of dissimilatory nitrate reduction, accounting for 28.3% and 37.9% of the variance in DNF and DNRA rates, respectively. High algal biomass accelerated DNF rates, while indirectly affected DNRA via changing the quality of organic carbon. The greater contributions of DNRA to dissimilatory nitrate reduction were found in lakes with higher sulfate concentrations. DNRA coupled to sulfur cycling may play an important role in lakes with high sulfate concentrations and high sediment organic carbon. This study highlights the important role played by DNRA in total nitrate reduction pathways of freshwater lakes. Mitigation strategies for N pollution and algal blooms should not only target decrease of nutrient input, strategies should also create a suitable environment for improving N removal and inhibit N recycling.