Abstract
Dengue virus was detected for the first time in Chile, in an outbreak of dengue fever on Easter Island. The virus was isolated in tissue culture and characterized by reverse transcription–polymerase chain reaction as being dengue type 1.
Highlights
Dengue fever (DF) is a common viral disease of the tropics
The samples were brought to our laboratory, and plasma was used for viral culture and for reverse transcription–polymerase chain reaction (RT-PCR)
The isolated dengue virus was identified as DENV-1 serotype by immunofluorescence antibody testing (IFAT) by using monoclonal antibodies in slides prepared from the viral cultures (Figure 1) and by RT-PCR obtaining a band of 482 bp (Figure 2)
Summary
Dengue fever (DF) is a common viral disease of the tropics. Only a few countries in the Americas, including Chile, have not reported cases of this disease. Before the outbreak on Easter Island, 15 cases of DF had been diagnosed in continental Chile in 2000 and 2001 and serologically confirmed in our laboratory.
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