Deneysel Model Çalışması: 1/2-5/6 Nefrektomize ve Diyabetik Ratlarda Böbreğin Histomorfolojik ve Ultrastrüktürel Değişiklikleri

Abstract

Objective: Diabetes and hypertension are often seen together and their prevalence shows large variability according to geographical, cultural, demographic, nutritional and genetic factors. These diseases affect the kidneys directly or indirectly. In this study, our aim is to compare of the effect of diabetes on kidney histomorphologically and by electron microscopic evaluation using two models of the different variations of the remnant kidney model as in surgical unilaterally nephrectomized (1/2Nx) and subtotal nephrectomized (5/6Nx) rats. Material and Methods: For this aim, six groups were created: Control, Diabetes mellitus (DM), 1/2 (right) nephrectomy, 5/6 nephrectomy, 1/2 (right) nephrectomy+DM and 5/6 nephrectomy+DM. Kidney tissues were analyzed using histomorphometric and ultrastructural analysis. Results: Renal damage has showed a very rapid progression with DM and hypertension. In 1/2 Nx group with unilateral nephrectomy, the renal damage progression is not rapid as in 5/6 Nx group, but glomerular injury increases with diabetes. Scar areas, Bowman capsule thickness, nuclei/glomerulus and glomerular diameter in DM and hypertension groups were significantly higher than control and 1/2 nephrectomy groups. 5/6 Nx+Diabetes group observed the most rapid progress overall in both light and electron microscopic measurements. Conclusion: Glomerular damage has showed a very rapid progression together with diabetes and hypertension.We think that our results wouldsupply important histomorphometric and ultrastructural data and contribute to the literature especially for the model studies of hypertension and diabetes, together and separately.