Deneysel Kolit Modelinde Centella Asiatika Ekstresinin Etkinliği

Abstract

Background : Inflammatory bowel disease (IBD) is a group of chronic and inflammatory conditions that are considered to be caused by environmental, genetic, and immune factors. The treatment of IBD mainly involves combinations of anti-inflammatory, immunosuppressive, and cytotoxic agents. However, due to serious side effects of these agents, research is being performed to find new treatment methods. Methods: The 24 rats were divided into three groups. In control group, the rats received drinking water and standard rat chow, in colitis group, the rats received drinking water with DSS, and in CA+colitis group the rats were administered 200 mg/kg/day CA and then received drinking water with DSS. Histopathologic examination, concentrations of myeloperoxidase (MPO), malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and interleukin-10 (IL-10), were determined. Results: The results indicated that the TNF-α level was lower and the IL-10 was higher in the rats administered with CA extract compared to the rats in the colitis group ( p <0.05). The MPO and MDA levels were significantly higher in the colitis group compared to other groups ( p <0.05). Histopathologic examination revealed less tissue damage in the rats administered with CA extract. Conclusion : The CA extract exerts an immunoregulatory effect by altering the levels of anti- and pro-inflammatory cytokines in experimental IBD models. It also prevents tissue damage by inhibiting the formation of free oxygen radicals. Depending on these effects, we consider that the CA extract can be a useful agent in the treatment of IBD.