Dendropanax morbifera L\xe9veille extract ameliorates d-galactose-induced memory deficits by decreasing inflammatory responses in the hippocampus

Abstract

In the present study, we examined the effects of Dendropanax morbifera Léveille leaf extract (DML) on dgalactose-induced morphological changes in microglia and cytokines, including pro-inflammatory cytokines (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α) and anti-inflammatory cytokines (IL-4 and IL-10) in the hippocampus. Administration of DML to d-galactose-treated mice significantly improved d-galactose-induced reduction in escape latency, swimming speed, and spatial preference for the target quadrant. In addition, administration of DML to d-galactose-treated mice significantly ameliorated the microglial activation and increases of IL-1β, IL-6, and TNF-α levels in the hippocampus. Administration of d-galactose significantly reduced IL-4 levels in the hippocampus, while administration of DML to d-galactose-treated mice significantly increased IL-4 level. However, we did not observe any significant changes in IL-10 levels in hippocampal homogenates. These results suggest that DML reduces d-galactose-induced mouse senescence by reducing pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α, as well as increasing anti-inflammatory cytokine IL-4.