DENDROBENTHAMIDIA CAPITATA (WALL.) HUTCH. FRUIT (BHOMORA): AN OPULENT SOURCE OF POLYPHENOLS WITH ANTIOXIDANT, ANTI-ELASTASE, ANTI-COLLAGENASE, AND ANTI-TYROSINASE ACTIVITIES FROM INDIAN HIMALAYAN REGION

Abstract

Dendrobenthamidia capitata (Wall.) Hutch. fruit (Bhamora) is a popular yet underexplored wild edible fruit of Uttarakhand state of Indian Himalayan region. Therefore, the present study was aimed to analyze D. capitata fruit for its phenolic contents, antioxidant, anti-elastase, anti-collagenase, and anti-tyrosinase activities using in- vitro assays. Phenolics and flavonoids were extracted using 80 % aqueous acetone and acid acetone as solvents. Our analysis revealed high values of total phenolic contents of 4.38±0.08 mg gallic acid equivalents (GAE). g-1 fruit weight and total flavonoid contents of 7.47±0.66 mg catechin equivalents (CE). g-1 fruit weight in the acid acetone fruit extracts of D. capitata. D. capitata fruit extracts demonstrated remarkable antioxidant activities (ABTS+, 1,1- diphenyl-2-picrylhydrazyl, superoxide anion, linoleate peroxyl radicals scavenging, and ferric reducing activities) and notable anti-elastase, anti-collagenase, and anti-tyrosinase activities. High resolution liquid chromatography– mass spectroscopy analysis revealed presence of six phenolic compounds, namely, dihydrorobinetin, dhydromyricetin, retusin dimethyl ether, rotenone, bergenin (C-glycoside of 4-O-methyl gallic acid) and peucenin in fruit extracts. This study recommends utilization of D.capitata fruit as functional food with prospective pharmaceutical, nutraceutical, and cosmeceutical properties.