Abstract
BackgroundTargeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. RNAs that have over-representation in the dendritic fraction were annotated and sorted into function groups.ResultsWe have identified 154 dendritic mRNA candidates, which can be arranged into the categories of receptors and channels, signaling molecules, cytoskeleton and adhesion molecules, and factors that are involved in membrane trafficking, in protein synthesis, in posttranslational protein modification, and in protein degradation. Previously known dendritic mRNAs such as MAP2, calmodulin, and G protein gamma subunit were identified from our screening, as were mRNAs that encode proteins known to be important for synaptic plasticity and memory, such as spinophilin, Pumilio, eEF1A, and MHC class I molecules. Furthermore, mRNAs coding for ribosomal proteins were also found in dendrites.ConclusionOur results suggest that neurons transport a variety of mRNAs to dendrites, not only those directly involved in modulating synaptic plasticity, but also others that play more common roles in cellular metabolism.
Highlights
Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity
Proteins can be made in the cell body and transported to synapses, local synthesis of proteins from dendritic mRNAs provides an efficient mechanism for delivering synaptic proteins where and when they are needed
Our rationale is that since most mRNAs are restricted to the cell body, an mRNA that is targeted to the dendrite should constitute a higher proportion of the total RNA extracted from dendritic laminas than that from cell body layers
Summary
Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. Proteins can be made in the cell body and transported to synapses, local synthesis of proteins from dendritic mRNAs provides an efficient mechanism for delivering synaptic proteins where and when they are needed. It has become evident that mRNAs are transported to the dendrite and translated locally near the synapse following the discovery of synapse-associated polyribosome complexes (SPRCs) [1], and local protein synthesis has been demonstrated following synaptic activation [2,3,4,5,6,7,8]. Enlarged views of the microarrays are presented showing reproducible hybridization
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