Abstract
In hepatitis C virus (HCV) infection dendritic cells (DCs) are a key player in immune response and modulation. Therefore they are considered a promising tool for viral defence. After 8 day maturation with GMCS-F and IL–4 bone-marrow derived DCs from BALB/c mice were loaded and therefore activated by hepatitis C virus pseudo particles (HCVpp). HCVpp consists of the two envelope proteins E1 and E2 (in our case genotype 1b), covering a non-HCV core structure. Mice were vaccinated two times by intra peritoneal injection of the activated DCs. After four weeks mice were sacrificed and ELISPOT and ELISA assays were performed. As target antigen synthetic peptides were used, covering the whole two envelope proteins. So we identified epitopes responsible for immune response of dendritic cells when infected with HCV. If the single epitope is responsible for immune reaction or just in combination with others has further to be evaluated.
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