Dendritic branching features of posterodorsal medial amygdala neurons of adult male and female rats: Further data based on the Golgi method

Abstract

The posterodorsal portion of the medial amygdalar nucleus (MePD) contains receptors for gonadal hormones and modulates the function of a social behavior network in rodents. The aims of this study were: to provide further data about the morphology of Golgi-impregnated dendrites of neurons from the MePD of adult rats; and, to compare the results obtained for dendritic branching and predominant dendritic spatial distribution in the MePD of males and diestrus females. MePD neurons were classified as bitufted or stellate, their spiny dendrites showed variable lengths, divided sparingly and decreased the number of branches with the distance from the soma. Dendritic arborization levels, number of branches in each level, distribution of the dendrites around the cell body and away from it, and the preferred spatial distribution of dendritic branches were studied according to different techniques and compared between sexes. Statistically significant differences were found in the predominant dendritic spatial distribution in the MePD, males with branches more oriented medially and dorsolaterally and females with more dorsally and ventromedially ones (p< or =0.05 in all cases). This result adds another clue to understand how information is processed and integrated in the MePD and within functionally dynamic sex steroids-responsive circuits relevant for reproduction in both sexes.