Dendritic and synaptic properties of collicular neurons: A quantitative light and electron microscopical study of Golgi‐impregnated cells

Abstract

The present study deals with a light- and electron microscopic morphometric analysis of Golgi-impregnated neurons in the superior colliculus of rats with the purpose to unravel inter- and intralaminar differences in their dendritic and synaptic organization. In particular, layer IV was studied and compared with its boundary layers III and V. The results show that collicular cells in layer IV basically form a homogeneous population with respect to the number of primary dendrites, the total length of impregnated dendrites, and the diameter, ellipticity, and orientation of dendritic fields and somata of Golgi-impregnated neurons. Somata of reconstructed small cells in layer III and IV as well as V have all a similar density of about 40 synaptic contacts per 100 microns2 surface. However, the cell bodies of large multipolar cells in layer V have a slightly but significantly larger synaptic density (about 50 per 100 microns2). Dendrites of large and small collicular cells had no significantly different synaptic densities (43 and 48 per 100 microns2, respectively). In conclusion, the present results show only minor dendritic and synaptic differences between individual cells in the same layer, as well as in neighboring layers, which implies a low degree of cellular and synaptic intra- and interlaminar differentiation. It is discussed that this organization differs markedly from that in other visual centers, including the collicular homologue, the tectum of lower vertebrates, and the mammalian visual cortex, where pronounced inter- and intralaminar differentiations exist. Such an organization may provide a framework of laminar specificity by which distinct cell types may select a restricted set of input out of all information available. The present quantitative investigation suggests that a similar framework is not present in the superior colliculus.