Abstract
BackgroundThe exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.MethodsWe synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer.ResultsThe results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection.ConclusionOur findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor.
Highlights
The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics
Synthesis and characterization of the au Gold nanorods (NRs)@Poly(amidoamine) dendrimers (PAMAM)‐GX1 Cetyltrimethylammonium bromide (CTAB)-coated Au NRs were synthesized by a seed-mediated growth method
The Au NR@PAMAM-GX1 was prepared by GX1 with Au NR@PAMAM
Summary
The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. Traditional cancer treatments, such as surgery, chemotherapy and radiotherapy, have severe side effects. Long-term chemotherapy can lead to tumor resistance to multiple drugs, making cancer treatment difficult. Moveover, killing cancer cells in the same way can cause damage to healthy tissues or organs, causing side effects such as insomnia, vomiting, loss of appetite and leukopenia [1, 2]. PTT is an effective method for the treatment of tumors due to its low cost, good local treatment effect and little side effects [3,4,5]. For photothermal therapy of tumors, different nanoparticles have been designed and synthesized including carbon nanotubes [6], graphene oxide [7], and gold nanoparticles
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