Abstract
The effect of chloramphenicol (CAP) on the initiation and continuation of DNA repair in X-irradiated Micrococcus radiodurans was studied. The repair of single strand breaks proceeds by two separate mechanisms, one of which is resistant to CAP, proceeds without thymidine incorporation, and is inhibited by EDTA. This direct repair might be due to the direct action of polynucleotide ligase on breaks with 3′OH and 5′PO4 end groups. The other type of repair is the classical repair replication: this requires postirradiation protein synthesis to be initiated but once under way is not halted by CAP.Postirradiation incubation with EDTA irreversibly prevented subsequent direct repair. The hypothesis is advanced that unrepaired breaks were converted to gaps by enzymatic activity during incubation with EDTA.
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