Abstract

Bovine papilloma virus and virus DNA isolated from ten cases of bovine fibropapillomatosis were shown to fall into two distinct classes. The virus classes shared up to 52–58% of their polynucleotide sequences as assayed by saturation hybridization and 45% by analysis of DNA-DNA reassociation kinetics, with heteroduplexes being less thermally stable than homoduplexes. The viruses also differed in their ability to hemagglutinate mouse erythrocytes and were antigenically cross-reactive but not identical by complement fixation and hemagglutination inhibition. The electrophoretic mobilities of structural polypeptides in sodium dodecyl sulfate-polyacrylamide gels were similar, with only minor differences being observed.

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