Abstract
We demonstrate an extended depth of focus optical coherence tomography (OCT) system based on the use of chromatic aberration to create displaced focal planes in the sample. The system uses a wavelength-swept source tuning over three spectral bands and three separate interferometers, each of which interfaces to a single illumination/collection fiber. The resulting three imaged volumes are merged in post-processing to generate an image with a larger depth of focus than is obtained from each band individually. The improvements are demonstrated in structural imaging of a porous phantom and a lipid-cleared murine brain, and by angiographic imaging of human skin. By using a coaxial approach with Gaussian beams, this approach enables an extended focus with relatively simple microscope optics and data-merging algorithms.
Highlights
Optical coherence tomography (OCT) is an imaging modality based on low coherence interferometry [1]
We have demonstrated a system that leverages chromatic aberration to extend the depth of focus (DoF) of OCT
The shift in focal planes was confirmed by measurement of the transverse point-spread function (PSF), and its use to extend DoF was presented in a sponge phantom, an angiographic imaging of human skin, and a cleared murine brain tissue
Summary
Optical coherence tomography (OCT) is an imaging modality based on low coherence interferometry [1]. These methods require a sample phase-stability that is difficult to achieve in many settings In this manuscript, we demonstrate an approach for multi-focus imaging using triband illumination. We engineered the chromatic aberration of the microscope optics to cause a displacement in the focal plane of each band such that imaging data across all three bands can be merged to create a single extended-focus acquisition. This approach has the advantage of maintaining a single-fiber microscope with Gaussian beam illumination and detection, while simplifying the fusion of the imaging data by using coaxial beams
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