Abstract

A highly sensitive radioassay for tryptic activity was used to measure acrosin in mouse and rabbit spermatozoa. The assay showed acrosin activities for rabbit spermatozoa similar to those published by others while the specific activities in mouse spermatozoa were ten-fold lower. Inhibitor studies showed that this residual amount of trypsin-like activity could not be inhibited by tryptic inhibitors which have an effect on rabbit spermatozoa. It is concluded that mouse spermatozoa do not have significant amounts of acrosin.

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