Abstract
ABSTRACTMolecular sensing using stimuli-responsive viscoelastic property of amphiphilic polymer hydrogel was demonstrated. Thermo-responsive poly(N-isopropylacrylamide); NIPA, hydrogel immobilizing bovine serum albumin (BSA) in its polymer chain was synthesized on the AT-cut QCM (Quartz crystal microbalance). The device was provided to the sensing of anti-BSA antibody. The resonance behavior of QCM in response to the adsorption of anti-BSA antibody on the hydrogel was observed based on both the resonance frequency and resistance of QCM with impedance analysis. The QCM device coated with the LCST polymer hydrogel revealed a characteristic resonance behavior in response to the adsorption of anti-BSA antibody in the collapse phase of gel at which the gel lost most of its hydrated water. The resonance frequency and resistance in the collapsed gel were highly sensitive and their changes in response to the target adsorption were much larger than in the swelling state of gel. The use of the device at the LCST of NIPA gel is ideal because the largest amplification of the adsorption signal is available along with the largest phase change of NIPA gel.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
