Demonstration of Infectious Taura Syndrome Virus in the Feces of Seagulls Collected during an Epizootic in Texas

Abstract

Laughing gulls Larus atricilla were observed feeding on diseased shrimp Penaeus vannamei during an epizootic of the Taura syndrome virus (TSV) at a south Texas farm in May 1995. Fresh fecal samples were collected from a gull roosting site on a pond levee where gulls that fed on diseased shrimp had roosted overnight. The feces samples were tested for the presence of TSV by injection bioassay. A cell-free clarified homogenate of the fecal material was injected into indicator P. vannamei, and the shrimp were observed for 9 d. Cumulative mortalities of the two replicate groups of indicator shrimp were 45% and 70%. Moribund indicator shrimp collected and preserved for histological examination exhibited lesions pathognomonic for acutephase infections by TSV. The presence of TSV in the induced lesions was confirmed with in situ hybridization with cDNA probes specific for a portion of the TSV genome. The presence of infectious TSV in the gulls' feces supports the hypothesis that these birds are a probable transport vector of the virus within and among nearby shrimp farms.