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Abstract
Hagemann1 reported the superiority of a fluorescence method using berberine sulfate stain for demonstrating leprosy bacilli in nasal mucus and thick blood smears. The same year he2 proposed an auramin-fluorescence technic for the routine identification of acid-fast fast bacteria, especially tubercle bacilli. The suitability of this method for cultures of Myco. leprae has been confirmed by Kuster3 and for leproma smears by Kline and Leach.4Recently one of us (H.J.H.) has investigated the separation and concentration of acid-fast organisms from the tissues of leprosy patients. To cryochemed spleen† distilled water was added and direct impression smears were made from a piece of the tissue. One set of smears was stained with auramin O and examined according to the fluorescence procedure described by Richards and Miller.5A representative field is shown in the accompanying photomicrographs. The faint material in the background is non-acid-fast splenic cellular substance. The acid-fast bacilli have a characteri...
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