Abstract
1. 1. Cobamide coenzyme was extracted from the body wall of adult female Ascaris suum maintained in Ellison's medium containing vitamin B 12. 2. 2. The identification of the coenzyme in purified fractions was based on (a) specific spectral changes following treatment of the preparation with light and cyanide, (b) coenzyme activity in the glutamate mutase reaction and (c) inactivation of the coenzyme activity by light. 3. 3. A fivefold increase in coenzyme occurred in worms maintained in Ellison's medium plus vitamin B 12, compared to the endogenous value. The data indicate that vitamin B 12 is converted by A. suum into the metabolically active coenzyme.
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