Abstract
Using crude extracts from the nitrogen-fixing blue-green alga, Anabaena cylindrica, a decarboxylation reaction can be demonstrated, which is dependent on pyruvate and ferredoxin and which is stimulated by coenzyme A, a SH-protecting compound and ATP. The activity of the pyruvate decarboxylation in Anabaena grown with ammonia is lower than one-fifth of that in cells grown with molecular nitrogen or nitrate as the nitrogen source. From this it is suggested that a physiological role of the reaction is to generate reduced ferredoxin which serves as the reductant in the assimilation of nitrogen to ammonia.
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