Demographic characteristics, risk factors and immunocytochemistry of p<sup>16INK4a</sup>, Ki-67, MCM5, and survivin as predictors for the progress of cervical precancer lesion

Abstract

Aim To identify the predictive factors and biomarkers in the progression of cervical precancer lesion or Cervical intraepithelial Neoplasia (CIN). Methods The study was conducted from August 2007 to September 2008. Design of the study was case-control with stratifications of test dose response. The cases were patients with CIN. Control patients were non CIN patients. Bivariate analysis followed by multivariate analysis was conducted. Results There were 130 patients, consisting of124 CIN patients divided into CIN 1, CIN 2 and CIN 3, with the following numbers of patients: 30, 41, and 33, respectively and 26 patients without CIN (non CIN). Bivariate analysis showed that age 13 years, sexual partner z 2, first sexual relationship at age 2 persons, positive HPV DNA, and over expression ofp 16INK4a, Ki-67 and Survivin that showed a P value of < 0.005. Conclusion Younger ages, education age > 13 years, sexual partner > 2 persons, positive HPV DNA, high p161NK4a, Ki-67 and Survivin expression constituted the risk factors for the occurrence ofthe progress of CIN, and was used in the equation to predict the progress of cervical precancer lesion. (Med J lndones 2010; 19:147-53)