Demineralized freeze-dried bovine bone xenograft enhance osteoblast viability and proliferation for jaw regeneration materials

Abstract

Objectives The aim of this study was to analyze the viability and proliferation properties of the DFDBBX in MC3T3-E1 cell line Material and Methods Bovine was milled to reach nano sizes of 100-200 nm. We used 2 mg/mL, 4 mg/mL and 8 mg/mL to incubate MC3T3-E1 cells in the following experiment. Immunocytochemistry methods were performed using a Live/Dead cell imaging kit to investigate the viability of MC3T3-E1. To investigate the proliferation of MC3T3-E1, ICC was performed using an anti-Ki67 antibody. Using a confocal laser scanning microscope (CLSM), the cells were observed at LSIH Brawijaya University. Statistical analysis was performed using the one-way analysis of variance (ANOVA) at p < 0.05 Results The highest intensity of living cells was DFDBBX 8 mg/ml on day 7 (739.46 A.U.; p < 0.05), indicating that DFDBBX 8 mg/ml had an effect of increasing cell viability two times compared to control. Ki67 intensity in MC3T3-E1 cell culture was highest on day seven and treated DFDBBX 8 mg/ml (186.04 A.U.; p< 0.05), which indicated the proliferation of MC3T3-E1 cells increased nine times compared to the first day and two times greater than the control group Conclusion DFDBBX have great viability and proliferation properties, which shows potentially as biomaterials to repair bone loss