Abstract
Precipitation of ferrocyanide ions by ferric ions (the Prussian blue reaction) has provided one of the few methods used for direct visualization of tissue channels under the electron microscope. The technique is improved by use of tris(ethylenediamine)cobalt(III) chloride as the precipitating salt. Inclusion of this salt in the primary fixative, unlike the acidic ferric salts normally included, allows fixation to occur at physiological pH. The results show that tissues are well preserved, and both the position and size of the precipitate deposits are consistent with what is presently known.
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