Abstract
An additional new pigment exhibiting anthocyanin and flavone disubstituted malonate structure, was isolated from the blue-purple flowers of Eichhornia crassipes as a minor anthocyanin. This pigment was identified as (6'''-O-(delphinidin 3-O-(6''-O-(β-D-glucopyranosyl)-β-D-glucopyranosyl))) (6''-O-(luteolin 7-O-(β-D-glucopyranosyl))) malonate by chemical and spectroscopic methods. The λmax of this pigment is 547 nm in 0.1% HCl-MeOH, where the negative cotton effect is also observed as similar to that of Eichhornia anthocyanin A, (delphinidin 3-gentiobiosyl) (apigenin 7-glucosyl) malonate previously isolated from the same flowers. This result supports that the new pigment also takes a folding conformation between delphinidin (chromophore) and luteorin (co-pigmnet).
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