Abstract
Objectives: The aim of this study was to evaluate the antioxidant, anti-inflammatory, and anti-cancer potencies of the Delonix regia bark, a first of its kind.
 Methods: The bark was extracted sequentially in Soxhlet apparatus with hexane, chloroform, and methanol in the increasing order of polarity. These extracts were subjected to find its antioxidant activity and total phenol content. Antibacterial activity against human pathogenic bacteria was tested. The anti-inflammatory properties were elucidated by its capacity to inhibit 15-lipoxygenase (LOX) and human cyclooxygenase (COX)-2. Cell cytotoxic capacity was evaluated against MCF-7 cells breast cancer cell lines.
 Results: Liquid chromatography (LC)-Mass Spectroscopy (MS) fingerprint of the methanol extract identified a total of 14 polyphenols, of which five were structurally characterized based on their mass-charge ratio [M-H]− peak, UV-vis absorption in comparison to published data. Antibacterial activity by disk diffusion inhibited human pathogenic bacteria. Bacterial biofilm inhibition capacity of extract (750 mg) imaged by confocal laser scanning microscopy revealed loss of microcolonies. Extract when tested for 15-LOX inhibition exhibited IC50 values of 94.5 ± 1.23 mg.mL−1 by enzyme kinetics studies using spectrophotometric techniques. Similarly, it could inhibit COX-2 enzyme at relatively lower concentrations (32.18 ± 1.91 mg.mL−1). Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay indicating its strong antioxidant property with the capacity to protect DNA. In vitro cytotoxicity was evaluated by 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphynyl tetrazolium bromide assay and apoptosis induced in MCF-7 cells was assessed morphologically.
 Conclusion: Our data suggest that D. regia bark methanol extract exerts its therapeutic activity for further pharmaceutical evaluations. Further studies are necessary to determine the mechanisms of these pharmacological properties.
Highlights
The Liquid chromatography (LC)-chromatogram obtained is shown in Fig. 1, and peaks were labeled according to the order of their retention time
DPPH and ABTS radical scavenging assay The percentage of DPPH decolorization is attributed to hydrogen donating ability of test compounds
Minimum inhibitory concentration (MIC) These extracts were assessed for their efficiency on the bacteria by MIC analysis by broth microdilution method
Summary
Ex. Hook) Raffin (Family: Fabaceae Caesalpinioideae) (synonyms – Poinciana regai, Bojer ex Hook.), is known to common man as Gul mohar, It is a medium sized ornamental tree grown in parks, gardens, and road side and has yellow-brown wood. Hook) Raffin (Family: Fabaceae Caesalpinioideae) (synonyms – Poinciana regai, Bojer ex Hook.), is known to common man as Gul mohar, It is a medium sized ornamental tree grown in parks, gardens, and road side and has yellow-brown wood It is called a flamboyant or flame ornamental tree. Some of the properties reported for bark include relieving knee pain, emetic effect, as bio-monitors, and bio-accumulants of atmospheric trace metals. The bark, fruits, and leaves have been used as anti-helminthic, reducing rheumatic pain and as purgatives in Pirojpur district, Bangladesh. The people of Yanadi (a tribal community in Andhra Pradesh, India) used flowers of Delonix regia in the treatment of dysmenorrhea [2,3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
