Abstract
DNAzyme (DZ) compared to conventional antisense oligonucleotides have significant potential capacity in the treatment of cancer because of high selectivity and intrinsic catalytic efficiency. Considering the key role of c-Myc oncogene in the progression of breast cancer, herein the β-CD polymer (β-CDP) is used to delivery of RNA-cleaving DZ which causes suppression of the c-Myc gene in MCF-7 cell line. The best suppression efficiency in the MTT viability assay, in a time and concentration-dependent manner obtained 24 h after transfection of 0.8 μM of the β-CDP/DZ complex (weight ratio 1:1). Results of Real-time-qPCR showed 1.7 fold decrease the expression of c-Myc mRNA after cell transfection of 0.8 μM inclusion complex. By flow cytometry analysis, apoptosis rate of cancer cells was high (45.6%), with a significant difference compared to control. Also synergistic effect of the β-CDP/DZ complex and different concentration of doxorubicin (DOX) was investigated. Results showed, inhibitory effect of cell proliferation of DOX in the presence of β–CDP/DZ complex is better than the free drug. Results suggested a potential platform in association with therapeutic potential of RNA-cleaving DZ for c-Myc oncogene inhibition in cancer therapy and indicated extremely efficient at delivery of β–CDP/DZ polyplex in the MCF-7 cell line.
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