Abstract
We demonstrate a modular and versatile experimental setup that enables straightforward compression (and then shaping) of ultrashort laser pulses at the imaging plane of a two-photon microscope. A commercially available pulse shaper is used in conjunction with a commercially available broadband Ti:Sapphire oscillator to produce sub-8fs pulses at the focus of a high-numerical-aperture objective. Automated adaptive pulse compression, based on multiphoton intrapulse interference phase scan (MIIPS), is verified in situ by shaper assisted interferometric autocorrelation. Two-photon excited fluorescence image of a mouse kidney slide is obtained to confirm microscopic sectioning capabilities.
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