Abstract
BackgroundMurraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid content by spectrophotometric methods. The UHPLC technique was employed for profiling the different polyphenolic compounds present in the extract/fractions. Furthermore, the extract/fractions were analysed for the antioxidant and antimutagenic activities using different in vitro assays.ResultsIt was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g. Likewise, UHPLC method also showed maximum number as well as amount of polyphenolic compounds in ethyl acetate fraction. It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays. In Ames assay, ethyl acetate fraction again showed maximum antimutagenic activity of 97.70 % against the 2-aminofluorene mutagen in TA100 tester strain of Salmonella typhimurium.ConclusionOverall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen. The maximum bioactivities of the ethyl acetate fraction may be linked to the presence of high number of polyphenolic compounds as shown by spectrophotometric as well as UHPLC methods.
Highlights
Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and known for its ethnobotanical importance
Many mutagens may act through the endogenous generation of reactive oxygen species which interact with the nucleotides, disulphide bonds and polyunsaturated fatty acids
These extract/fractions were further subjected to UHPLC analysis for the profiling and quantification of polyphenolic compounds which was performed on Nexera UHPLC (Shimadzu) system equipped with C-18 Column
Summary
Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and known for its ethnobotanical importance With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. Many mutagens may act through the endogenous generation of reactive oxygen species which interact with the nucleotides, disulphide bonds and polyunsaturated fatty acids These interactions support the execution of oxidation process of biological compounds and act as initiators of various degenerative diseases such as cardiovascular, emphysema, inflammatory diseases, Kaur et al SpringerPlus (2016) 5:1037 cataracts etc. The antioxidant rich medicinal plants as known to be the reservoir of bio-protective compounds, exerts their effects to scavenge the free radicals via inducing phase II enzymes (Devi et al 2015). Efforts are needed to explore the medicinal plant for the presence of protective compounds (Zahin et al 2013)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
