Delignifizierung und Enzymbehandlung von ultradünnen Holzschnitten

Abstract

Ultrathin sections of spruce and beech wood produced from samples embedded in water-soluble melamine resin (“Nanoplast”) have proved very stable during chemical and enzymatic treatment processes. They are therefore well suited to the study of the delignification progress or of other processes involving cell wall degradation at TEM resolution. Using successive sections the effects of treatment on the cell walls can be compared with the original condition in the same tissue area or even in the same cell.